第59期：日糧添加三丁酸甘油酯對成年短尾母羊的瘤胃微生物蛋白質(zhì)產(chǎn)量，發(fā)酵特性及...

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作者：Qing-Chang Ren1，Jing-Jing Xuan1，Li-Ke Wang1，Qiu-Wen Zhan1，Ding-Zhong Yin1，Zhong-Ze Hu1，Hong-Jian Yang2，Wei Zhang2，Lin-Shu Jiang3

1Anhui Science and Technology University,Fengyang, China

2State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University (CAU), Beijing,China

3Beijing Key Laboratory of Dairy CowNutrition, Beijing University of Agriculture,Beijing, China

來源：Anim Sci J. 2018;1–9.DOI: 10.1111/asj.13033

翻譯：腸動力研究院 梁琦

摘 要

本研究旨在通過體外和體內(nèi)試驗(yàn)來評估日糧添加三丁酸甘油酯（TB）對成年短尾母羊的瘤胃微生物蛋白質(zhì)產(chǎn)量和發(fā)酵特性的影響。在體外試驗(yàn)中，選取9只瘤胃插管的短尾母羊（12月齡，55±5kg），日糧以干物質(zhì)（DM）為基礎(chǔ)，同時添加不同濃度的TB（0,2,4,6和8g / kg），二者混合48h，采食過后收集9只羊的瘤胃液用于分析TB對羊瘤胃養(yǎng)分降解率和發(fā)酵特性的影響。而在體內(nèi)試驗(yàn)中，45只成年母羊（12月齡，55±5kg）根據(jù)初始體重被隨機(jī)分配成5個試驗(yàn)組，分飼DM基礎(chǔ)日糧+不同濃度的TB（0,2,4,6和8g / kg），每組9只，試驗(yàn)為期18天。實(shí)驗(yàn)結(jié)果顯示，體外試驗(yàn)下，日糧添加TB顯著增強(qiáng)了成年母羊?qū)M（p=0.009），粗蛋白（p <0.001），中性洗滌纖維（p=0.007）和酸性洗滌纖維（p=0.010）的降解和利用，同時瘤胃的產(chǎn)甲烷能力相應(yīng)增加（p <0.001）。而體內(nèi)試驗(yàn)則表明，日糧添加TB雖然顯著降低了成年母羊?qū)M的攝入量（p <0.001），但它顯著增加了瘤胃微生物蛋白質(zhì)的生成（p <0.001）。總之，體外和體內(nèi)試驗(yàn)均表明日糧添加TB可使羊瘤胃內(nèi)總揮發(fā)性脂肪酸（VFA）的濃度和纖維素酶（木聚糖酶，羧甲基纖維素酶，結(jié)晶纖維素酶）的活性得到相應(yīng)的增加。研究結(jié)果表明，日糧添加TB可對羊瘤胃微生物蛋白質(zhì)的產(chǎn)量和發(fā)酵特性產(chǎn)生積極的影響。

關(guān)鍵詞：成年母羊；甲烷；微生物粗蛋白；三丁酸甘油酯；揮發(fā)性脂肪酸

以下為相關(guān)圖表

表1：基礎(chǔ)日糧的組成及化學(xué)成分

表2：與干物質(zhì)基礎(chǔ)日糧混合48h后，三丁酸甘油酯對體外發(fā)酵特性的影響

表3：體外試驗(yàn)下，三丁酸甘油酯對干物質(zhì)（DM），粗蛋白（CP），中性洗滌纖維（NDF）和酸性洗滌纖維（ADF）表觀降解率的影響

表4：體外試驗(yàn)下，與干物質(zhì)混合48小時后，三丁酸甘油酯對瘤胃產(chǎn)氣特性的影響

表5：體內(nèi)和體外試驗(yàn)下，三丁酸甘油酯對纖維素酶活性的影響

表6：三丁酸甘油酯對成年短尾母羊的日干物質(zhì)攝入量和瘤胃內(nèi)發(fā)酵特性的影響

表7：三丁酸甘油酯成年短尾母羊瘤胃內(nèi)微生物繁殖的影響

結(jié) 論

雖然體外試驗(yàn)未能減少產(chǎn)甲烷作用，但日糧添加三丁酸甘油酯（TB）對總VFA濃度，纖維素酶活性，發(fā)酵效率和微生物蛋白質(zhì)合成具有有利影響，TB可能對成年母羊的生產(chǎn)性能產(chǎn)生積極的影響。

Abstract

Two trials were conducted to assess the effects of tributyrin (TB) supplementation on ruminal microbial protein yield and fermentation characteristics in adult sheep. In an in vitro trial, substrate was made to offer TB at 0, 2, 4, 6, and 8 g/kg on a dry matter (DM) basis and incubated for 48 hr. In an in vivo trial, 45 adult ewes were randomly assigned by initial body weight (55±5 kg) to five treatments of nine animals over an 18-day period. Total mixed ration was made to offer TB to ewes at 0, 2, 4, 6, and 8 g/kg on a DM basis. The in vitro trial showed that TB enhanced apparent degradation of DM (p = .009), crude protein (p < .001), neutral detergent fiber (p = .007) and acid detergent fiber (p = .010) and increased methanogenesis (p < .001), respectively. The in vivo trial showed that TB decreased DM intake (p < .001) and enhanced rumen microbial N synthesis (p < .001), respectively. Both in vitro and in vivo trials showed that TB increased total volatile fatty acid concentration and enhanced fibrolytic enzyme activity. The results indicated that TB might exert positive effects on microbial protein yield and fermentation in the rumen.

Conclusion

Although the in vitro trial failed to decrease methanogenesis, substrate and feed supplementation of TB showed advantageous effects on total VFA concentration, fibrolytic enzyme activity, fermentation efficiency and microbial protein synthesis, which might have positive effects on performance in adult ewes

原創(chuàng)： 梁琦      轉(zhuǎn)自：腸動力